1. Bring the card and Balance to room
temperature. Label the card with patient's / donor's name or identification
number properly.
2. Remove the foil seal to be ready for
testing.
3. Dispense the sample to the saline to
prepare a 1% red blood cell saline suspension. Add 50μl of this
suspension into the microtubes.
4. Add 50μl serum or plasma
into the microtubes.
5. Flick the test card a few times if
necessary, make the reactant thoroughly mixed
6. Incubate the card for 15-25 minutes in
the 37℃ incubator.
7. After incubation, Centrifuge the gel
card(s) in the dedicated Centrifuge at 1500rpm for 4 minutes.
8. After centrifugation, remove the gel
card(s) from the centrifuge. Observe, read macroscopically the front and back
of each microtube for agglutination and/or hemolysis and record reactions.
Quality Control
The User is recommended to choose the
suitable control for Lab daily quality control according to the requirement of
specificity and sensitivty in this instruction.
Quality control selection principle is as
follows:
Negative Control: Using serum or plasma
without irregular antibodies, or using non-sensitized red blood cell. Do the
indirectly coombs test. The non-sensitized red blood cell means the red blood
cell is negative in the direct coombs test. The serum or plasma of AB type
blood can be regarded as the serum or plasma without irregular antibodies
Positive control: Using the serum or plasma
with IgG antibodies, and the red blood cell positive to the corresponding
antigen, for example, using the 64 times diluted anti-D(IgG) and RhD-positive
cells. Do the indirectly coombs test. observe the results is positive or not,
and the intensity of positve reaction.
